miR-4458 regulates cell proliferation and apoptosis through targeting SOCS1 in triple-negative breast cancer

Area of Science:

• Oncology
• Molecular Biology
• Gene Regulation

Background:

• Suppressor of cytokine signaling 1 (SOCS1) is implicated in triple-negative breast cancer (TNBC) progression, affecting cell proliferation and apoptosis.
• Databases suggest SOCS1 is a direct target of microRNA-4458 (miR-4458).

Purpose of the Study:

• To investigate the relationship between miR-4458 and SOCS1 in TNBC.
• To elucidate the functional role of miR-4458 in TNBC pathogenesis.

Main Methods:

• Comparative analysis of miR-4458 expression in TNBC tissues/cells versus normal controls.
• Validation of direct binding between miR-4458 and SOCS1.
• Assessment of miR-4458's impact on SOCS1 expression (mRNA and protein).
• Evaluation of miR-4458's effects on cell proliferation and apoptosis in TNBC cells.
• Correlation analysis of miR-4458 levels with clinical TNBC staging.

Main Results:

• miR-4458 expression was significantly decreased in TNBC tissues and cells.
• miR-4458 directly targets and negatively regulates SOCS1 expression at both mRNA and protein levels.
• miR-4458 suppressed proliferation and promoted apoptosis in TNBC cells, mediated by SOCS1.
• Lower miR-4458 levels were observed in advanced TNBC clinical stages compared to early stages.

Conclusions:

• miR-4458 functions as a tumor-suppressive microRNA in TNBC.
• The tumor-suppressive role of miR-4458 is achieved through the regulation of SOCS1.
• miR-4458 may serve as a potential biomarker for TNBC progression.