Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Imaging01:24

X-ray Imaging

10.1K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
10.1K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

7.2K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
7.2K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.5K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.5K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.3K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.3K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

800
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
800
X-ray Crystallography02:18

X-ray Crystallography

26.1K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
26.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Homologous supramolecular confinement in a cyano-functionalized MOF enables rapid Li<sup>+</sup> transport in composite polymer electrolytes.

Chemical communications (Cambridge, England)·2026
Same author

Divergent regulation of DREB1A by ICE1 and ABF4 contributes to distinct cold adaptation in four turfgrass species.

Journal of advanced research·2026
Same author

Programmed cryogenic fabrication of cuttlebone-inspired lightweight cellular materials with enhanced energy absorption.

Nature communications·2026
Same author

Unveiling the potential regulatory functions of long non-coding RNAs in potato tuberization through integrated analysis of transcriptomics and phytohormone profiles.

BMC plant biology·2026
Same author

Metallothionein-Inspired Dual-Stage Ion-Regulatory Coatings With Infection-Triggered Bactericidal Activity and Long-Term Antifouling Protection.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

The Big Data Science Center at the Shanghai Synchrotron Radiation Facility: the architecture of the superfacility.

Journal of synchrotron radiation·2026

Related Experiment Video

Updated: Jan 27, 2026

X-ray Dose Reduction through Adaptive Exposure in Fluoroscopic Imaging
08:30

X-ray Dose Reduction through Adaptive Exposure in Fluoroscopic Imaging

Published on: September 11, 2011

14.9K

Three-dimensional focal stack imaging in scanning transmission X-ray microscopy with an improved reconstruction

Limei Ma, Xiangzhi Zhang, Zijian Xu

    Optics Express
    |March 17, 2019
    PubMed
    Summary

    This study introduces an improved focal stack (FS) algorithm for 3D imaging in X-ray microscopy. The new method efficiently detects multiple focused objects, overcoming limitations of previous algorithms for clearer 3D reconstructions.

    More Related Videos

    Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
    08:04

    Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

    Published on: March 12, 2017

    9.8K
    Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
    14:23

    Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

    Published on: March 6, 2018

    11.4K

    Related Experiment Videos

    Last Updated: Jan 27, 2026

    X-ray Dose Reduction through Adaptive Exposure in Fluoroscopic Imaging
    08:30

    X-ray Dose Reduction through Adaptive Exposure in Fluoroscopic Imaging

    Published on: September 11, 2011

    14.9K
    Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
    08:04

    Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

    Published on: March 12, 2017

    9.8K
    Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
    14:23

    Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

    Published on: March 6, 2018

    11.4K

    Area of Science:

    • Microscopy
    • Imaging Science
    • Computational Imaging

    Background:

    • Focal stack (FS) is vital for fast 3D imaging in high-resolution scanning transmission X-ray microscopy.
    • Accurate object localization along the optical axis is critical for effective FS reconstruction.
    • Previous algorithms suffer information loss by detecting only one focused object per optical-axial pixel line (OAPL).

    Purpose of the Study:

    • To develop an improved focal stack algorithm for enhanced 3D imaging.
    • To address the limitation of single-object detection in previous FS reconstruction methods.
    • To enable more accurate and comprehensive 3D imaging of sparse samples.

    Main Methods:

    • Developed an improved focal stack algorithm.
    • Utilized a calculated threshold for normalized local variances.
    • Extracted multiple focused objects from each optical-axial pixel line (OAPL).

    Main Results:

    • Demonstrated the feasibility and high efficiency of the improved FS algorithm.
    • Successfully extracted multiple focused objects per OAPL.
    • Validated through simulation and experimental results for 3D imaging.

    Conclusions:

    • The improved FS algorithm effectively overcomes previous information loss issues.
    • The method is highly efficient for 3D imaging of high-contrast, sparse samples.
    • This advanced approach shows potential for complex sample 3D X-ray microscopy.