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Related Experiment Videos

Methods for extracting 'omes from microbialites.

Elizabeth Selene Gómez-Acata1, Carla M Centeno2, Luisa I Falcón1

  • 1Laboratorio de Ecología Bacteriana, Instituto de Ecología, Universidad Nacional Autónoma de México, CdMx 04510, Mexico; Unidad Académica de Yucatán, UNAM, Parque Científico y Tecnológico de Yucatán, Sierra Papacal, Yucatán 97302, México.

Journal of Microbiological Methods
|March 17, 2019
PubMed
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Extracting genetic material from microbialites is challenging due to their composition. This review details DNA and RNA extraction methods, including multi-omic approaches, for microbialite research.

Area of Science:

  • Geomicrobiology
  • Molecular Biology
  • Biochemistry

Background:

  • Microbialites are complex organo-sedimentary structures built by microbial communities.
  • High carbonate content and exopolymeric substances in microbialites hinder biomolecule extraction.
  • Existing DNA and RNA extraction methods often require harsh chemicals or mechanical disruption.

Purpose of the Study:

  • To review and compare various DNA and RNA extraction methods for microbialites.
  • To discuss the advantages and disadvantages of different extraction techniques.
  • To explore advanced multi-omic and viral extraction strategies for microbialites.

Main Methods:

  • Review of published literature on microbialite biomolecule extraction.
  • Analysis of chemical and mechanical lysis techniques.
Keywords:
MetagenomicsMetatranscriptomicsMicrobialiteMultiomicsViral extractionXanthogenate

Related Experiment Videos

  • Discussion of commercial DNA/RNA extraction kits.
  • Exploration of multi-omic (MPlex) and viral extraction protocols.

    • Main Results:

    • Various chemical agents (e.g., CTAB, SDS) and mechanical methods are used for lysis.
    • Commercial kits offer convenience but may require optimization for microbialites.
    • Multi-omic approaches enable comprehensive analysis of microbialite communities.
    • Virus extraction from microbialites remains an emerging area.

    Conclusions:

    • Effective DNA and RNA extraction from microbialites requires careful method selection.
    • Multi-omic techniques are crucial for a holistic understanding of microbialite ecosystems.
    • Further research is needed to optimize viral extraction from these complex matrices.