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Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
Published on: February 7, 2013
Effect of polyhexamethylene biguanide functionalized silver nanoparticles on the growth of Staphylococcus aureus
Jialin Yi1, Yuelin Zhang1, Wenshu Lin1
1School of Life Sciences, Shanghai University, Shanghai 200444, PR China.
Abstract:
Polyhexamethylene biguanide (PHMB) was used as a stabilizing ligand to synthesize uniform silver nanoparticles (Ag NPs). The effects and action mechanism of PHMB functionalized Ag NPs (Ag NPs-PHMB) on the growth of the Gram-positive bacteria Staphylococcus aureus were investigated. The results showed that a high concentration Ag NPs-PHMB could be obtained and prepared particles were fairly uniform. Prepared Ag NPs-PHMB enhanced the bactericidal effect and the log kill reached 5.06 when S. aureus was exposed to Ag NPs-PHMB for 20 min. Ag NPs-PHMB caused damage to cell wall, a decrease in the membrane fluidity, and leakage of K+, Mg2+, ATP and proteins from the cell, eventually leading to the death of S. aureus.
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