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Updated: Jan 27, 2026

Author Spotlight: Exploring Cloning Techniques for Full-Length DNA Fragments
Published on: May 17, 2024
Establishment of a rescue system for porcine parvovirus using a seamless cloning method
Lingling Zhang1,2, Dou Gao1,2, Yongle Yu3
1Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Abstract:
In this study, we describe a novel and rapid method for the construction of a full-length infectious clone (pPPV). The constructed clone contained an engineered EcoRv site that served as a genetic marker and was shown to be infectious when transfected into a monolayer of PK-15 cells. The rescued virus (rPPV) of the infectious clone was found to be indistinguishable from wild-type virus BQ in terms of its biological properties. The generation of this PPV infectious clone provides a potentially powerful tool with which to elucidate the molecular pathogenesis of PPV.
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