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Photochemically crosslinked cell-laden methacrylated collagen hydrogels with high cell viability and functionality.

Thuy-Uyen Nguyen1, Kori E Watkins1, Vipuil Kishore1

  • 1Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, Melbourne, Florida, 32901.

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Summary

VA086 is a more biocompatible photoinitiator than Irgacure 2959 for crosslinking collagen hydrogels, supporting better cell viability and osteoblast function in tissue engineering.

Keywords:
I2959Saos-2 cellsVA086methacrylated collagenphotochemical crosslinking

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Photochemistry

Background:

  • Irgacure 2959 (I2959) is a common photoinitiator for hydrogel crosslinking but generates cytotoxic free radicals.
  • Methacrylated collagen (CMA) hydrogels are promising for tissue engineering but require effective and cytocompatible crosslinking methods.

Purpose of the Study:

  • To compare the biocompatibility and efficacy of VA086 versus I2959 as photoinitiators for CMA hydrogel crosslinking.
  • To investigate the impact of photoinitiator type, concentration, and crosslinking time on hydrogel properties and encapsulated cell behavior.

Main Methods:

  • Photochemical crosslinking of CMA hydrogels using I2959 and VA086 under varying conditions.
  • Characterization of hydrogel morphology (SEM), mechanical properties (compression tests), and stability.
  • Assessment of Saos-2 cell viability (live-dead assay), metabolic activity, and osteogenic differentiation (Alizarin Red S staining).

Main Results:

  • Photochemical crosslinking reduced hydrogel porosity and water retention but enhanced stability.
  • Increasing photoinitiator concentration improved compressive modulus.
  • VA086-crosslinked hydrogels showed significantly higher cell viability and enhanced cell-mediated mineralization compared to I2959-crosslinked hydrogels.
  • Saos-2 cells maintained osteoblast functionality in VA086-crosslinked hydrogels.

Conclusions:

  • VA086 is a more cytocompatible photoinitiator than I2959 for creating CMA hydrogels for tissue engineering.
  • VA086 supports superior cell viability and osteogenic function, making it a preferred choice for biomaterial applications.
  • Optimized photochemical crosslinking conditions are crucial for balancing hydrogel properties and biological performance.