Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.7K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.7K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

4.1K
4.1K
Sanger Sequencing01:57

Sanger Sequencing

774.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
774.3K
Next-generation Sequencing03:00

Next-generation Sequencing

98.4K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
98.4K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

12.8K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
12.8K
Per-Unit Sequence Models01:26

Per-Unit Sequence Models

443
An ideal Y-Y transformer, grounded through neutral impedances, displays per-unit sequence networks akin to those of a single-phase ideal transformer when subjected to balanced positive- or negative-sequence currents. These currents do not produce neutral currents, and their associated voltage drops.
Zero-sequence currents, which are identical in magnitude and phase, generate a neutral current, resulting in voltage drops across the neutral impedance and the low-voltage winding. If the...
443

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same author

Regulation of reproductive and behavioral plasticity in social insects.

Current opinion in insect science·2026
Same author

A Lineage-Specific Peptide Suppresses Juvenile Hormone to Drive Reproductive and Longevity Reprogramming in Ants.

bioRxiv : the preprint server for biology·2026
Same author

Modern Mining: The Role of Single-cell RNA Sequencing in Advancing Neuroscience Research.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same author

Spatial, temporal and Notch determination of terminal selector expression controls neuronal cell fate in the Drosophila optic lobe.

Nature neuroscience·2026
Same author

Gene expression cartography of a developing neuronal structure.

Developmental biology·2026

Related Experiment Video

Updated: Jan 27, 2026

Detection of Copy Number Alterations Using Single Cell Sequencing
09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

12.1K

Neuro-evo-devo in the single cell sequencing era.

Nikos Konstantinides1, Sophie Degabriel1, Claude Desplan1,2

  • 1Department of Biology, New York University, New York, NY 10003, USA.

Current Opinion in Systems Biology
|March 20, 2019
PubMed
Summary

Investigating the evolution of the nervous system is complex. New single-cell sequencing and CRISPR technologies enable comparisons across species to understand neuronal development and evolution.

More Related Videos

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing
05:34

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Published on: February 10, 2023

2.3K
Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy
04:21

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

Published on: January 19, 2024

3.5K

Related Experiment Videos

Last Updated: Jan 27, 2026

Detection of Copy Number Alterations Using Single Cell Sequencing
09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

12.1K
Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing
05:34

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Published on: February 10, 2023

2.3K
Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy
04:21

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

Published on: January 19, 2024

3.5K

Area of Science:

  • Evolutionary biology
  • Neuroscience
  • Developmental biology

Background:

  • The nervous system is the most complex tissue in animals.
  • Understanding the evolution of this complexity is a significant scientific challenge.

Purpose of the Study:

  • To leverage recent technological advancements to study neuronal evolution.
  • To identify key developmental factors driving the evolution of new cell types.

Main Methods:

  • Single-cell sequencing techniques to analyze cell populations.
  • Algorithmic clustering to identify distinct cell types.
  • Developmental trajectory analysis to map cell lineages.
  • CRISPR genome editing for validation of regulatory elements.

Main Results:

  • New technologies facilitate the comparison of homologous cell types across species.
  • Identification of critical developmental stages and transcription factors involved in cell-type evolution.
  • CRISPR technology allows for experimental validation of predicted evolutionary regulators.

Conclusions:

  • The convergence of single-cell genomics and genome editing offers unprecedented opportunities to investigate neuronal evolution.
  • Future research will likely yield significant insights into how complex nervous systems evolve and develop.