Nanocrystalline cellulose decorated quantum dots based tyrosinase biosensor for phenol determination

Area of Science:

• Materials Science
• Electrochemistry
• Environmental Science

Background:

• Phenol contamination poses environmental and health risks.
• Development of sensitive and selective biosensors is crucial for phenol detection.
• Nanocomposite materials offer unique properties for biosensor development.

Purpose of the Study:

• To develop a novel biosensor for accurate phenol determination.
• To utilize a nanocrystalline cellulose (NCC)/cadmium sulfide quantum dots (CdS QDs) nanocomposite scaffold.
• To immobilize tyrosinase enzyme for enhanced electrocatalytic activity.

Main Methods:

• Fabrication of a CTAB-modified NCC decorated with 3-MPA capped CdS QDs.
• Immobilization of tyrosinase enzyme onto the NCC/CdS QDs nanocomposite.
• Electrochemical evaluation using differential pulse voltammetry (DPV).

Main Results:

• The Tyr/CTAB-NCC/QDs nanocomposite showed a proportional current response to phenol concentration.
• The biosensor exhibited good linearity in the 5-40 μM range (R² = 0.9904).
• Achieved sensitivity of 0.078 μA/μM and a limit of detection (LOD) of 0.082 μM.

Conclusions:

• The developed biosensor architecture is effective for sensitive phenol detection.
• The NCC/CdS QDs nanocomposite provides a suitable scaffold for enzyme immobilization.
• This biosensor holds promise for environmental monitoring of phenolic pollutants.