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Determination of Total Lipid and Lipid Classes in Marine Samples
Published on: December 11, 2021
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Validation Including Uncertainty Estimation of a GC⁻MS/MS Method for Determination of Selected Halogenated Priority
Slávka Nagyová1, Peter Tölgyessy2
1Water Research Institute, Slovak National Water Reference Laboratory, Nábrežie arm. gen. L. Svobodu 5, 812 49 Bratislava, Slovak Republic. slavka.nagyova@vuvh.sk.
Foods (Basel, Switzerland)
|March 21, 2019
Summary
A new rapid method efficiently determines organochlorines and polybrominated diphenyl ethers in fish. This green analysis approach uses modified QuEChERS and GC-MS/MS, offering simplicity and cost-effectiveness.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
Background:
- Persistent organic pollutants (POPs) like organochlorines (OCs) and polybrominated diphenyl ethers (PBDEs) accumulate in fish, posing risks to ecosystems and human health.
- Accurate and efficient analytical methods are crucial for monitoring these contaminants in food sources.
Purpose of the Study:
- To develop and validate a rapid, cost-effective, and environmentally friendly method for determining specific OCs and PBDEs in fish samples.
- To assess the method's performance using standard reference materials and real fish samples.
Main Methods:
- A modified QuEChERS (quick, easy, cheap, effective, rugged and safe) sample preparation involving pH-tuned dispersive liquid-liquid microextraction (DLLME).
- Sulfuric acid (H₂SO₄) digestion for fish extract clean-up.
- Gas chromatography-triple quadrupole tandem mass spectrometry (GC-QqQ-MS/MS) for analysis.
Main Results:
- The method demonstrated good linearity, recovery, and accuracy, validated against NIST SRM 1946.
- Successful application to real fish samples from eight different species.
- The analytical Eco-Scale evaluation classified the method as an acceptable green analysis method.
Conclusions:
- The developed method is simple, rapid, cost-effective, and provides high extract clean-up efficiency and good sensitivity.
- It offers significant advantages over existing QuEChERS-based methods for OC and PBDE determination in fish.
- The method is suitable for routine monitoring of these contaminants in fish.
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