Related Experiment Video
Updated: Jan 27, 2026

Simultaneous Cryosectioning of Multiple Rodent Brains
Published on: September 18, 2018
Simultaneous extraction and analysis of multiple cystine-dense peptides by μSPE and microflow-MS/MS from plasma
Mi-Youn Brusniak1, Chad Christianson2, Emily Witthuhn2
1Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, Seattle, WA 98109, USA.
Abstract:
Aim: Develop a universal extraction and liquid chromatography-mass spectrometer method to simultaneously analyze cystine-dense peptide (CDP) miniproteins from rat and human plasma. The results of the analysis will be used to assist selection of therapeutic drug candidates from the vast CDP library. Methods & results: A micro-elution solid-phase extraction method was developed for the sample preparation of the CDP peptides in rat and human plasma followed by analysis by microflow liquid chromatography MS/MS. The methods developed for drug discovery were found to be accurate (±≤15.2% from nominal concentrations) and precise (≤13.4% CV), with a dynamic range of 1.00-500 ng/ml and extraction recoveries of 47.2-99.0%. Conclusion: This bioanalytical method can be utilized to screen CDP proteins and other miniproteins for drug discovery, candidate selection and further drug development.
More Related Videos
06:18Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis
Published on: February 21, 2019
06:12Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods
Published on: April 14, 2023
Related Concept Videos
Peptide Bonds
Dense Connective Tissue
Dense Regular Connective Tissue
In dense regular connective tissue, fibers are arranged parallel to each other, enhancing its tensile strength and resistance to stretching in the direction of the fiber orientations. Ligaments and tendons are made of dense regular...
Multiple Allele Traits
Multiple Regression
Farmers can use multiple regression to determine the crop yield based on more than one factor, such as water availability, fertilizer, soil properties, etc. Here, the crop yield is the response or dependent variable as it depends on the other independent variables. The analysis requires the construction of a scatter plot...
Composition of Blood Plasma
Deformation of Member under Multiple Loadings
In the case of a member with a variable cross-section, the strain is not constant but depends on the position. The deformation of an...