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Updated: Jan 27, 2026

Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis
Published on: January 30, 2018
Overview of RAW264.7 for osteoclastogensis study: Phenotype and stimuli
Lingbo Kong1, Wanli Smith2, Dingjun Hao1
1Department of Spine, School of Medicine, Honghui-hospital, Xi'an Jiaotong University, Xi'an, China.
Abstract:
Bone homeostasis is preserved by the balance of maintaining between the activity of osteogenesis and osteoclastogenesis. However, investigations for the osteoclastogenesis were hampered by considerable difficulties associated with isolating and culturing osteoclast in vivo. As the alternative, stimuli-induced osteoclasts formation from RAW264.7 cells (RAW-OCs) have gain its importance for extensively osteoclastogenic study of bone diseases, such as rheumatoid arthritis, osteoporosis, osteolysis and periodontitis. However, considering the RAW-OCs have not yet been well-characterized and RAW264.7 cells are polymorphic because of a diverse phenotype of the individual cells comprising this cell linage, and different fate associated with various stimuli contributions. Thus, in present study, we provide an overview for current knowledge of the phenotype of RAW264.7 cells, as well as the current understanding of the complicated interactions between various stimuli and RAW-OCs in the light of the recent progress.
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