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Updated: Jan 27, 2026

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Multidomain P450 Epoxidase and a Terpene Cyclase from the Ascochlorin Biosynthetic Pathway in Fusarium sp
Zhiyang Quan1, Takayoshi Awakawa1,2, Dongmei Wang1,3
1Graduate School of Pharmaceutical Sciences , The University of Tokyo , Bunkyo-ku, Tokyo 113-0033 , Japan.
Abstract:
Ascochlorin is a medicinally important fungal meroterpenoid. Its biosynthetic pathway in Fusarium sp. was identified, and the stereoselective epoxidation of the farnesyl group by the multidomain, soluble P450 monooxygenase AscE and the subsequent formation of the unique timethylcyclohexanone ring by the membrane-bound cyclase AscF were investigated. Precursor-directed biosynthesis generated novel bromo-substituted derivatives, which exhibited potent cytotoxic activities. This study paves the way for the future metabolic engineering of medicinally important meroterpenoids for drug discovery.
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