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A feedback loop involving MdMYB108L and MdHY5 controls apple cold tolerance
Yicheng Wang1, Zuolin Mao1, Huiyan Jiang1
1National Key Laboratory of Crop Biology, College of Horticulture Science, Shandong Agricultural University, Tai-An, Shandong, China.
Biochemical and Biophysical Research Communications
|March 24, 2019
Summary
This study identifies a new apple gene, MdMYB108L, that boosts cold tolerance by activating MdCBF3. It also reveals a feedback loop where MdMYB108L downregulates MdHY5, enhancing plant survival under cold stress.
Area of Science:
- Plant Molecular Biology
- Plant Physiology
- Genetics
Background:
- MYB transcription factors play crucial roles in plant stress responses.
- Understanding cold tolerance mechanisms in apples is vital for agriculture.
Purpose of the Study:
- To identify and characterize a novel apple MYB gene involved in cold stress response.
- To elucidate the regulatory pathway of cold tolerance mediated by MdMYB108L and MdHY5.
Main Methods:
- Gene isolation and identification of MdMYB108L in apple.
- Analysis of transgenic apple calli overexpressing MdMYB108L.
- Yeast one-hybrid and electrophoretic mobility shift assays (EMSA).
Main Results:
- MdMYB108L expression is induced by light and cold stresses.
- Overexpression of MdMYB108L enhances cold tolerance by upregulating MdCBF3.
- MdMYB108L directly binds to the MdCBF3 promoter.
- MdHY5, an integrator of light and cold signals, is downregulated in transgenic calli.
- A feedback mechanism exists where MdMYB108L downregulates MdHY5 transcription.
Conclusions:
- MdMYB108L enhances apple cold tolerance through MdCBF3 upregulation.
- A novel regulatory pathway involving a feedback loop between MdMYB108L and MdHY5 controls apple cold tolerance.
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