A dual amplification electrochemical immunosensor based on HRP-Au@Ag NPs for carcinoembryonic antigen detection

A sensitive sandwich-type electrochemical immunosensor based on dual amplification strategy was constructed. The dual amplification strategy has been used secondary antibody(Ab₂)-horseradish peroxidase(HRP)-Au@Ag nanoparticles (Au@Ag NPs) for carcinoembryonic antigen(CEA) detection. Ab₂-HRP-Au@Ag NPs as dual amplification markers triggered the disproportionation of H₂O₂, which could facilitate the catalytic oxidation of hydroquinone to quinone(BQ). In addition, due to their large surface area and excellent conductivity, nitrogen-doped graphene were used as a platform to firmly assemble primary antibody (Ab₁). Above mentioned generated amout of BQ are corresponding to trace CEA, resulting in the highly electrochemical reduction signal. Under the optimal conditions, the linear range of CEA concentration was 0.0001-100 ng mL^-1, and the limit of detection (LOD) could be as low as 0.05 pg mL^-1. Importantly, the immunosensor also showed acceptable stability, reproducibility and selectivity.