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Asian isolates of Anaplasma phagocytophilum: Multilocus sequence typing.
Tatyana A Mukhacheva1, Daria R Shaikhova1, Sergey Y Kovalev1
1Laboratory of Molecular Genetics, Department of Biology, Ural Federal University, Lenin Avenue 51, Yekaterinburg, 620000, Russia.
Ticks and Tick-Borne Diseases
|March 25, 2019
Summary
This study reveals novel genetic diversity in Anaplasma phagocytophilum, the bacteria causing granulocytic anaplasmosis. New sequence types were identified in Russian ticks, expanding our understanding of this pathogen
Area of Science:
- Veterinary Microbiology
- Molecular Epidemiology
- Tick-borne Diseases
Background:
- Anaplasma phagocytophilum causes granulocytic anaplasmosis in humans and animals.
- It is transmitted by Ixodes ticks and is widespread in Eurasia and North America.
- Multilocus sequence typing (MLST) is the standard for studying genetic diversity in A. phagocytophilum.
Purpose of the Study:
- To investigate the genetic diversity of A. phagocytophilum in Asian Russia.
- To compare MLST typing with 16S rRNA gene sequencing.
- To update the global A. phagocytophilum MLST database with new findings from Asia.
Main Methods:
- Collected 25 A. phagocytophilum isolates from Ixodes ticks in Asian Russia.
- Employed multilocus sequence typing (MLST) targeting seven housekeeping genes.
- Modified primers for six loci due to observed variability.
- Compared results with 16S rRNA gene typing.
Main Results:
- Identified 15 new sequence types (STs) and 39 new alleles, none matching existing database entries.
- Russian isolates clustered into two distinct groups (cluster 1 and 3), forming separate clades.
- For the first time, A. phagocytophilum isolates from Ixodes persulcatus and I. pavlovskyi ticks were found in cluster 3.
Conclusions:
- The study significantly expands the known genetic diversity of A. phagocytophilum in Asia.
- New MLST data from Russian isolates necessitate database updates and highlight regional genetic variations.
- Further research is required to determine the prevalence of these MLST clusters in tick and host populations across Asia.
Keywords:
16S rRNA typingAnaplasma phagocytophilumGenetic diversityIxodes ticksMultilocus sequence typingMore Related Videos
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