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Updated: Jan 27, 2026
Author Spotlight: Streamlining Composite Plant Production via Agrobacterium rhizogenes-Mediated Hairy Root Transformation
Published on: June 30, 2023
Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes
Abhijit A Daspute1,2,1,2, Xian Yunxuan1,3,1,3, Minghua Gu3,3
1Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
Aim:
To develop a useful alternative approach to evaluate the gene function in hairy roots.
Methods:
Arabidopsis and tobacco (wild-type or mutant) were a host for Agrobacterium rhizogenes transformation.
Results:
The hairy roots formation efficiency ranged from 53 to 98% in tobacco and 53 to 66% in Arabidopsis. Hairy and intact roots showed similar gene expression pattern in response to salt and aluminum stress. Genomic polymerase chain reaction and fluorescent images showed high rate (>80%) of co-integration of T-DNAs and uniform cell transformation without use of any antibiotic selection. Whole processes of hairy roots were completed within 1 month after the infection of Agrobacterium.
Conclusion:
Aluminum-responsive orthologous gene function could be evaluated by NtSTOP1-KD and Atstop1 as a host for hairy roots transformation.
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