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Related Experiment Video

Updated: Jan 21, 2026

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Published on: August 26, 2025

Genome Editing in Mice.

Lisbeth Ahm Hansen¹, Ernst-Martin Füchtbauer²

¹Department of Biomedicine, Aarhus University, Aarhus, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|March 27, 2019

Summary

This summary is machine-generated.

CRISPR/Cas9 gene editing in mouse zygotes is achievable via pronuclear injection or electroporation. These methods allow direct editing of the mouse genome at the earliest developmental stage.

Keywords:

CRISPR/Cas9 Electroporation Genome editing Mouse Pronucleus injection

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Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

Area of Science:

Molecular Biology
Genetics
Developmental Biology

Background:

CRISPR/Cas9 technology offers precise genome editing capabilities.
Efficiently editing the mouse genome in zygotes is crucial for genetic research and disease modeling.
Various methods exist for delivering gene-editing tools into zygotes.

Purpose of the Study:

To describe and compare methods for CRISPR/Cas9 mediated genome editing in mouse zygotes.
To highlight pronuclear injection and electroporation as effective techniques.
To provide an overview of alternative genome editing strategies in this context.

Main Methods:

Pronuclear injection of CRISPR/Cas9 components into mouse zygotes.
Electroporation of mouse zygotes with CRISPR/Cas9 reagents.
Review and mention of alternative genome editing delivery methods.

Main Results:

Successful genome editing in mouse zygotes was achieved using both pronuclear injection and electroporation.
These methods allow for direct modification of the mouse genome.
The study provides a practical guide to implementing these techniques.

Conclusions:

Pronuclear injection and electroporation are effective methods for CRISPR/Cas9 mediated genome editing in mouse zygotes.
These techniques facilitate the generation of genetically modified mice.
Further exploration of alternative methods may offer additional advantages.