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microRNA-448 inhibits the progression of non-small-cell lung cancer through regulating IRS2

Jie Gao1, Xiaojing Feng2, Fang Wang3

  • 1Department of CT, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei, China.

Journal of Cellular Biochemistry
|March 27, 2019
PubMed

Insights

MicroRNA-448 (miR-448) inhibits non-small-cell lung cancer (NSCLC) progression by suppressing insulin receptor substrates 2 (IRS2). Overexpression of miR-448 reduces NSCLC cell viability, metastasis, and epithelial-to-mesenchymal transition (EMT).

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNA-448 (miR-448) is implicated as a tumor-associated microRNA in various human cancers.
  • Understanding the role of miR-448 in non-small-cell lung cancer (NSCLC) is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the functional role of miR-448 in NSCLC progression.
  • To elucidate the relationship between miR-448 and insulin receptor substrates 2 (IRS2) in NSCLC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) to assess miR-448 and IRS2 expression.
  • MTT assay for cell viability.
  • Transwell and Western blot assays for cell metastasis and epithelial-to-mesenchymal transition (EMT).
  • Dual luciferase assay to confirm direct targeting of IRS2 by miR-448.

Main Results:

  • Downregulation of miR-448 and upregulation of IRS2 were observed in NSCLC.
  • miR-448 overexpression inhibited NSCLC cell viability, metastasis, and EMT.
  • miR-448 directly targeted IRS2, and IRS2 silencing also inhibited NSCLC progression.
  • Upregulation of IRS2 partially reversed the inhibitory effects of miR-448.

Conclusions:

  • miR-448 acts as a tumor suppressor in NSCLC.
  • Overexpression of miR-448 inhibits NSCLC proliferation, metastasis, and EMT by suppressing IRS2 expression.
  • Targeting the miR-448/IRS2 pathway may offer a therapeutic strategy for NSCLC.
Keywords:
IRS2epithelial-to-mesenchymal transitionmetastasismiR-448non-small-cell lung cancerproliferation

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