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Comparison of Fresh Feces with Lyophilized and Frozen Cultures of Feces as Inocula to Prevent Salmonella Infection in
H Pivnick1, B Blanchfield1, C Rigby1
1Bureau of Microbial Hazards, Food Directorate, Health Protection Branch, Department of National Health and Welfare, Ottawa, Ontario, Canada, K1A 0L2 and Agriculture Canada, Animal Disease Research Institute, Nepean P.O. Box 11300, Station H, Nepean, Ontario, Canada, K2H 8P9 and Division of Food Statistics and Operational Planning, Food Directorate, Health Protection Branch, Department of National Health and Welfare, Ottawa, Ontario, Canada, K1A 0L2.
Abstract:
Mixed treatment cultures obtained by inoculating anaerobic medium with chicken feces were administered to 0 to 1 d-old chicks in their drinking water. Three types of treatment cultures (all fourth passage) were compared: FFC (fresh fecal cultures started with inocula of fresh feces followed by four daily, uninterrupted serial subcultures), LFC (cultures started with inocula obtained by lyophilizing third-passage cultures), and FrFC (cultures started with inocula obtained by freezing third-passage cultures). Protection of treated chicks against infection by Salmonella typhimurium was assessed by challenging the chicks via their drinking water 2 d after treatment and culturing their ceca 8 to 9 d later, or at weekly intervals for 7 weeks. FFC protected chicks against infection more consistently than LFC or FrFC. However, some LFC and FrFC were as protective as FFC (α= .05). Treatment with cultures did not increase mortality or decrease weight gain.
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