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Related Experiment Video

Updated: Jan 27, 2026

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

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Published on: May 21, 2020

Divergent Switchgrass Cultivars Modify Cereal Aphid Transcriptomes.

Kyle G Koch^1,2, Erin D Scully³, Nathan A Palmer⁴

¹Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE.

Journal of Economic Entomology

|March 28, 2019

Summary

This summary is machine-generated.

Two aphid species, Schizaphis graminum and Sipha flava, show distinct molecular responses to switchgrass cultivars. Sipha flava better overcomes host defenses, indicating differential adaptation to bioenergy grasses.

Keywords:

Kanlow Summer de novo transcriptome assembly greenbug aphid yellow sugarcane aphid

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Area of Science:

Entomology
Molecular Biology
Bioenergy Crops

Background:

Schizaphis graminum and Sipha flava are generalist aphids feeding on bioenergy grasses.
These aphids exhibit differential colonization success on Panicum virgatum cultivars, with S. flava colonizing more cultivars than S. graminum.

Purpose of the Study:

To investigate the molecular responses of S. graminum and S. flava to feeding on two switchgrass (Panicum virgatum) cultivars.
To compare aphid transcriptional profiles on switchgrass with those on a sorghum host and under starvation conditions.

Main Methods:

De novo transcriptome assemblies were generated for S. graminum and S. flava.
Differential gene expression analysis was performed on aphids feeding on P. virgatum cv. Summer, P. virgatum cv. Kanlow, susceptible sorghum, and under starvation.

Main Results:

Transcriptome assemblies yielded 8,428 unigenes for S. graminum and 8,866 for S. flava.
S. graminum showed rapid upregulation of detoxification genes within 12 hours, while S. flava's major changes occurred at 24 hours.
Aphids downregulated growth and development genes on switchgrass, with divergent responses to Summer and Kanlow cultivars. S. flava upregulated more detoxification genes on Kanlow, suggesting better host defense evasion.

Conclusions:

S. graminum and S. flava exhibit distinct molecular strategies for coping with switchgrass defenses.
S. flava demonstrates a greater capacity to overcome host plant defenses, particularly on the Kanlow cultivar, aligning with its broader colonization ability.