Recognition of an HLA-DPw1 specific alloantiserum raised by planned immunization

This study reports identification of the first alloantiserum specific for a single HLA-DP allele and presents valuable technical information for the serological detection of the HLA-DP gene product. Serological detection of the HLA-DP gene products was undertaken by analyzing a large number of alloantisera against an HLA-DP characterized, monocyte depleted, B-lymphocyte reference panel. After absorption of contaminating DR antibodies, one alloantiserum (SOW) which had been raised by planned immunization, was shown to have a correlation coefficient of 0.91 with DPw1 as defined by primed lymphocytes. No association was seen with any other HLA-A,-B,-C,-D,-DR, or -DQ specificity in either population or family studies. The HLA-DP typing of the serum donor and immunizing recipient support the conclusion. Evidence is presented which suggests that expression of the DP molecule is not stable, at least as detected by conventional microcytotoxicity techniques.