A model for quantifying absorption through abnormal skin

Area of Science:

• Dermatology
• Pharmacokinetics
• Toxicology

Background:

• Quantitative absorption studies are established for normal skin.
• Limited data exists on abnormal skin permeability.
• Understanding abnormal skin absorption is crucial for drug delivery and safety.

Purpose of the Study:

• To develop and validate an in vivo model for quantifying chemical absorption through abnormal skin.
• To compare the permeability of normal and abnormal rat skin to model compounds.

Main Methods:

• An in vivo model was designed and evaluated for quantifying skin absorption.
• Absorption of [3H]mannitol and [14C]octyl benzoate was measured through altered rat skin.
• Comparison of absorption rates between normal and abnormal skin.

Main Results:

• [3H]Mannitol permeated normal skin significantly slower than [14C]octyl benzoate.
• Abnormal skin exhibited markedly increased permeability to both compounds.
• [3H]Mannitol absorption was over 100X greater, and [14C]octyl benzoate absorption was over 2X greater in abnormal skin compared to normal skin.

Conclusions:

• The developed in vivo model successfully quantifies absorption through abnormal skin.
• Abnormal skin demonstrates significantly enhanced permeability to tested compounds.
• This model provides a valuable tool for assessing dermal absorption in compromised skin conditions.