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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
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Mass Spectrometry: Isotope Effect01:13

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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
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MALDI-TOF Mass Spectrometry01:19

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
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Imaging Mass Spectrometry: A Perspective.

Richard M Caprioli1

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Journal of Biomolecular Techniques : JBT
|March 29, 2019
PubMed
Summary
This summary is machine-generated.

Imaging mass spectrometry (IMS) is a powerful tool for detecting molecules in complex samples. Matrix-assisted laser desorption ionization (MALDI) is a key technique within IMS, revealing molecular distributions in biological tissues and cells.

Keywords:
image fusionmolecular images

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Area of Science:

  • Analytical Chemistry
  • Biophysics
  • Molecular Imaging

Background:

  • Imaging mass spectrometry (IMS) offers broad molecular detection capabilities for complex biological samples.
  • Diverse ionization processes exist within IMS, with matrix-assisted laser desorption ionization (MALDI) being a major technique.

Purpose of the Study:

  • To highlight matrix-assisted laser desorption ionization (MALDI) as a significant ionization process within imaging mass spectrometry (IMS).
  • To emphasize the importance of IMS in providing insights into complex biological samples and spatial molecular distributions.

Main Methods:

  • Focus on matrix-assisted laser desorption ionization (MALDI) as a primary ionization technique in IMS.
  • Discussion of the application of IMS for analyzing molecular distributions at tissue and cellular levels.

Main Results:

  • IMS, particularly with MALDI, enables the visualization of molecular spatial distributions.
  • This technology provides critical insights into complex biological systems.

Conclusions:

  • IMS is a vital technology for biological and clinical research, enabling new discoveries.
  • Understanding molecular spatial distributions is crucial for fundamental biology and clinical applications.