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Bladder urinary oxygen tension is correlated with urinary microbiota composition
Megan B Shannon1, Roberto Limeira2, Danielle Johansen2
1Departments of Obstetrics & Gynecology and Urology, Stritch School of Medicine, Loyola University Chicago, 2160 S. First Ave, Maywood, IL, 60153, USA.
International Urogynecology Journal
|March 29, 2019
Summary
Researchers developed a new method to measure bladder urine oxygen tension (BUOT) in women. Lower oxygen levels were linked to urinary microbiota and symptoms like leakage.
Area of Science:
- Urology
- Microbiology
- Medical Technology
Background:
- Microbial communities in organ systems are influenced by environmental factors like oxygen availability.
- Understanding oxygen levels in the urinary tract is crucial for studying its microbiota.
Purpose of the Study:
- To introduce a novel technique for measuring bladder urine oxygen tension (BUOT) in ambulatory women.
- To investigate the relationship between BUOT, urinary microbiota, and urinary signs and symptoms in urogynecology patients.
Main Methods:
- A non-invasive flow-through oxygen sensor was used to determine BUOT in ambulatory female urogynecology patients undergoing transurethral catheterization.
- Urinary microbiota were identified using 16S rRNA gene sequencing of catheterized urine.
- Multivariate statistical analyses examined correlations between BUOT, microbiota composition, and clinical variables.
Main Results:
- Significant individual variation in BUOT was observed (median: 23.1 ± 13.5 mmHg).
- Microbiota composition was significantly associated with BUOT (p=0.03).
- Lower BUOT correlated with nitrite-negative urine (p=0.0001) and self-reported urinary leakage (p=0.01).
Conclusions:
- Bladder urine oxygen tension (BUOT) can be reliably measured in ambulatory women.
- A wide range of BUOT values exists among urogynecology patients.
- BUOT may be associated with the presence of urinary microbiota and related symptoms.
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