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Updated: Jan 27, 2026

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
Published on: January 16, 2018
Monitoring β-Arrestin 2 Targeting to the Centrosome, Basal Body, and Primary Cilium by Fluorescence Microscopy
Anahi Molla-Herman1,2, Kathryn M Davis3, Kirk Mykytyn4,5
1Department of Genetics and Developmental Biology, Institut Curie, CNRS-UMR3215, INSERM-U934, Paris, France. anahi.molla-herman@college-de-france.fr.
Abstract:
Primary cilia (PC) are microtubule-based organelles that behave like a cellular antenna controlling key signaling pathways during development and tissue homeostasis. The ciliary membrane is highly enriched for G protein-coupled receptors (GPCRs), and PC are a crucial signaling compartment for this large receptor family. Downstream effectors of GPCR signaling are also present in cilia, and evidence obtained by our labs and others demonstrated that β-arrestin (βarr) family members are differentially recruited to PC and have investigated the role of GPCR activation in this process. In this chapter, we provide methods based on fluorescence microscopy on fixed or live cells suitable for investigating targeting and recruitment of βarrs at PC.
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