Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Colloids03:22

Colloids

20.9K
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
20.9K
Colloids and Suspensions01:17

Colloids and Suspensions

3.3K
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles visible to the naked eye or seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. The suspended particles in a suspension settle out after some time of mixing. The separation of particles from a suspension is...
3.3K
Colloidal precipitates01:09

Colloidal precipitates

6.0K
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
6.0K
Local Anesthetics: Chemistry and Structure-Activity Relationship01:30

Local Anesthetics: Chemistry and Structure-Activity Relationship

6.5K
Local anesthetics (LAs) are drugs that induce a temporary loss of sensation in a limited body area, preventing pain. Cocaine was the first local anesthetic discovered in the late 19th century. Cocaine is a benzoic acid ester obtained from the leaves of coca shrubs and was often used for its psychotropic effects. Cocaine was first isolated in 1860 by Albert Niemann. Sigmund Freud studied the physiological actions of cocaine. Carl Koller later introduced it into clinical practice in 1884 as a...
6.5K
Protein Complex Assembly02:41

Protein Complex Assembly

16.7K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.7K
Acid–Base Equilibria: Activity-Based Definition of pH01:10

Acid–Base Equilibria: Activity-Based Definition of pH

1.2K
For an ideal solution, the pH is defined as the negative logarithm of the hydrogen ion concentration. For a non-ideal solution, an accurate measurement of the pH must consider the negative logarithm of the hydrogen ion activity rather than concentration. In such a solution, the pH can be more accurately defined as the negative logarithm of a product of the hydrogen ion concentration and its activity coefficient.
In solutions of very low ionic strength—for example, pure water—the...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Holographic Whole-Object Photopolymerization Preserving Director Alignment in Liquid Crystalline Actuators.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

All-optically controlled phased-array for ultrasonics.

Nature communications·2025
Same author

A roadmap for next-generation nanomotors.

Nature nanotechnology·2025
Same author

Massively parallel microbubble nano-assembly.

Nature communications·2025
Same author

Acoustic holographic assembly of cell-dense tissue constructs.

Biofabrication·2025
Same author

Low-power scalable multilayer optoelectronic neural networks enabled with incoherent light.

Nature communications·2024

Related Experiment Video

Updated: Jan 27, 2026

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

11.2K

Self-Assembled Phage-Based Colloids for High Localized Enzymatic Activity.

Mariana Alarcón-Correa1,2, Jan-Philipp Günther1,2, Jonas Troll1,2

  • 1Max Planck Institute for Intelligent Systems , Heisenbergstrasse 3 , 70569 Stuttgart , Germany.

ACS Nano
|March 29, 2019
PubMed
Summary
This summary is machine-generated.

Genetically engineered M13 bacteriophages on magnetic beads create highly active, biocompatible enzyme motors. This system enables efficient enzyme recovery and functions as a fast, localized enzymatic micropump, even in blood.

Keywords:
His tagbiohybrid nanostructuresenzymatic micropumpsenzyme recoveryphagesself-assembly

More Related Videos

Synthesis and Characterization of Supramolecular Colloids
09:26

Synthesis and Characterization of Supramolecular Colloids

Published on: April 22, 2016

10.4K
Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin
11:48

Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin

Published on: July 13, 2016

14.7K

Related Experiment Videos

Last Updated: Jan 27, 2026

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

11.2K
Synthesis and Characterization of Supramolecular Colloids
09:26

Synthesis and Characterization of Supramolecular Colloids

Published on: April 22, 2016

10.4K
Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin
11:48

Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin

Published on: July 13, 2016

14.7K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Biophysics

Background:

  • Catalytically active colloids are key for chemical motors and active matter research.
  • Current systems often rely on inorganic catalysts and toxic fuels, necessitating biocompatible alternatives.
  • Enzyme-coated colloids typically show lower activity compared to inorganic counterparts.

Purpose of the Study:

  • To develop a biocompatible system with high and localized enzymatic activity using engineered M13 bacteriophages.
  • To create a reusable enzyme immobilization platform with magnetic recovery capabilities.
  • To demonstrate the application of these enzyme-colloids as an efficient enzymatic micropump.

Main Methods:

  • Genetically engineered M13 bacteriophages were used to bind enzymes to magnetic beads, forming phage-decorated colloids.
  • The self-assembly process created a proteinaceous environment for directed enzyme immobilization.
  • Magnetic fields were employed for localized control and repeated recovery of the enzyme-colloids.

Main Results:

  • The phage-decorated colloids exhibited high and localized enzymatic activity.
  • Enzymatic activity was retained after repeated recovery of the enzyme-colloids using magnetic fields.
  • The system functioned as an enzymatic micropump, generating fluid flow, achieving the fastest reported flow for a biocompatible system.
  • The micropump operated effectively in complex media, including blood, utilizing physiological urea concentrations.

Conclusions:

  • Genetically engineered M13 bacteriophages facilitate efficient, localized enzyme immobilization on magnetic colloids.
  • This biocompatible system offers reusable enzymatic catalysts and demonstrates high performance as an enzymatic micropump.
  • The developed enzyme-phage-colloid system represents a significant advancement for active matter and microfluidic applications in biological environments.