High throughput automatic muscle image segmentation using parallel framework

Fast and accurate automatic segmentation of skeletal muscle cell image is crucial for the diagnosis of muscle related diseases, which extremely reduces the labor-intensive manual annotation. Recently, several methods have been presented for automatic muscle cell segmentation. However, most methods exhibit high model complexity and time cost, and they are not adaptive to large-scale images such as whole-slide scanned specimens.

In this paper, we propose a novel distributed computing approach, which adopts both data and model parallel, for fast muscle cell segmentation. With a master-worker parallelism manner, the image data in the master is distributed onto multiple workers based on the Spark cloud computing platform. On each worker node, we first detect cell contours using a structured random forest (SRF) contour detector with fast parallel prediction and generate region candidates using a superpixel technique. Next, we propose a novel hierarchical tree based region selection algorithm for cell segmentation based on the conditional random field (CRF) algorithm. We divide the region selection algorithm into multiple sub-problems, which can be further parallelized using multi-core programming.

We test the performance of the proposed method on a large-scale haematoxylin and eosin (H &E) stained skeletal muscle image dataset. Compared with the standalone implementation, the proposed method achieves more than 10 times speed improvement on very large-scale muscle images containing hundreds to thousands of cells. Meanwhile, our proposed method produces high-quality segmentation results compared with several state-of-the-art methods.

This paper presents a parallel muscle image segmentation method with both data and model parallelism on multiple machines. The parallel strategy exhibits high compatibility to our muscle segmentation framework. The proposed method achieves high-throughput effective cell segmentation on large-scale muscle images.