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Updated: Jan 27, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
Published on: May 13, 2019
Histone 2B monoubiquitination complex integrates transcript elongation with RNA processing at circadian clock and
Magdalena Woloszynska1,2, Sabine Le Gall1,2, Pia Neyt1,2
1Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
Histone monoubiquitination 1 (HUB1) and SPEN3 interact to regulate plant development. Their interaction links histone modification to pre-mRNA processing, impacting circadian clock and flowering time genes.
Area of Science:
- Plant molecular biology
- Epigenetics
- Gene regulation
Background:
- Histone monoubiquitination 1 (HUB1) and HUB2 are key components of a chromatin complex regulating plant development.
- HUB1 and HUB2 are involved in modulating developmental programs like flowering time, dormancy, and the circadian clock.
- RNA-binding proteins KHD1 and SPEN3 interact with HUB1 and HUB2.
Purpose of the Study:
- To investigate the functional relationship between HUB1, SPEN3, and their roles in chromatin-mediated gene regulation.
- To elucidate the molecular mechanisms by which HUB1 and SPEN3 influence circadian clock and flowering time.
- To understand how HUB1-mediated histone modification is linked to pre-mRNA processing.
Main Methods:
- Analysis of mutants in SPEN3 and KHD1 for phenotypic characterization (rosette, leaf area, flowering time).
- Luciferase reporter assays to assess circadian clock period.
- Transcriptomic analysis and double-mutant studies to investigate gene expression and interactions.
Main Results:
- SPEN3 and KHD1 mutants exhibit reduced plant size; spen3 mutants show delayed flowering, contrasting with hub1 mutants.
- HUB1 and SPEN3 are crucial for regulating CIRCADIAN CLOCK-ASSOCIATED1 (CCA1) and FLOWERING LOCUS C (FLC) expression.
- The HUB1-SPEN3 interaction connects histone H2B monoubiquitination (H2Bub) during transcript elongation with pre-mRNA processing at CCA1.
Conclusions:
- The study reveals a novel link between HUB1-mediated H2Bub and SPEN3-dependent pre-mRNA processing at CCA1.
- HUB1 is essential for SPEN3 function in producing FLC-derived antisense COOLAIR transcripts, influencing flowering time.
- The HUB1-SPEN3 complex plays a critical role in integrating epigenetic modifications with RNA processing for developmental control.
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