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Updated: Aug 11, 2026

07:36
Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
Published on: May 1, 2015
Single step isolation of immunofluorescence-stained, viable lymphocytes by fluorescence-activated cell sorter using
Abstract:
The paper presents a method for preparation of viable, immunofluorescence-stained lymphocytes in a single isolation step by fluorescence-activated cell sorter. Discrimination between viable, immunofluorescence positive cells and nonspecifically stained dead cells was carried out on the basis of the DNA-specific dye ethidium bromide. Ethidium bromide enters cells with damaged plasma membrane only. The method is described by the example of isolation of Lyt-2 positive mouse lymph node cells.

