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Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Accelerating structural life science by paramagnetic lanthanide probe methods.

Tomohide Saio1, Koichiro Ishimori1

  • 1Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan; Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, Hokkaido 060-8628, Japan.

Biochimica Et Biophysica Acta. General Subjects
|April 1, 2019
PubMed
Summary

Paramagnetic lanthanide ions enhance structural analysis in protein science using NMR and EPR spectroscopy. These probes offer precise distance and angular data, accelerating drug design and life science research.

Keywords:
Conformational changeElectron spin resonanceLigand screeningLong-range restraintNuclear magnetic resonanceParamagnetic lanthanide ionParamagnetic relaxation enhancementProtein structureProtein-ligand complexPseudocontact shiftResidual dipolar coupling

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Area of Science:

  • Biophysics and Structural Biology
  • Biochemistry and Molecular Biology

Background:

  • Paramagnetic lanthanide ions offer unique properties for structural analysis.
  • Nuclear Magnetic Resonance (NMR) and Electron Paramagnetic Resonance (EPR) spectroscopy are powerful tools for molecular structure determination.
  • Lanthanide binding tags facilitate the site-specific introduction of these paramagnetic probes.

Purpose of the Study:

  • To review recent advancements in structural analysis methods utilizing paramagnetic lanthanide ions.
  • To highlight the application of lanthanide probes in NMR and EPR spectroscopy.
  • To demonstrate the impact of these methods on protein science and drug design.

Main Methods:

  • Exploiting paramagnetic effects induced by trivalent lanthanide ions in NMR spectroscopy for long-range distance and angular information.
  • Utilizing paramagnetic lanthanide ions in EPR spectroscopy for nanometer-scale distance measurements.
  • Employing various lanthanide binding tags for targeted probe introduction.

Main Results:

  • Paramagnetic lanthanide ions provide long-range (~40 Å) distance and angular data in NMR.
  • Lanthanide probes enable nanometer-scale distance measurements in EPR.
  • Applications include protein structure determination, ligand screening, resonance assignment, and in-cell studies.

Conclusions:

  • Paramagnetic lanthanide probes significantly advance structural analysis in protein science.
  • These methods accelerate drug design and impact various areas of life science research.
  • The widespread use of lanthanide binding tags enhances the utility and accessibility of these techniques.