Testing thousands of nanoparticles in vivo using DNA barcodes

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Pharmacology

Background:

• Nanoparticles enhance drug efficacy by targeting disease sites.
• Predicting *in vivo* nanoparticle performance using *in vitro* methods is challenging due to physiological hurdles.
• Current methods limit the scale of nanoparticle evaluation *in vivo*.

Purpose of the Study:

• To discuss technologies for high-throughput *in vivo* screening of nanoparticles.
• To highlight DNA barcoded nanoparticles as a tool for efficient *in vivo* evaluation.
• To address the need for scalable methods in nanoparticle discovery pipelines.

Main Methods:

• Focus on technologies enabling high-throughput *in vivo* screening.
• Detailed discussion of DNA barcoded nanoparticles.
• Exploration of methods to overcome *in vivo* physical and physiological barriers.

Main Results:

• Identified technologies for large-scale *in vivo* nanoparticle assessment.
• Demonstrated the potential of DNA barcoded nanoparticles for efficient screening.
• Addressed the gap between *in vitro* testing and *in vivo* efficacy prediction.

Conclusions:

• High-throughput *in vivo* screening is crucial for effective nanoparticle drug delivery.
• DNA barcoded nanoparticles offer a promising solution for scalable *in vivo* evaluation.
• Advancing nanoparticle discovery pipelines requires integrated *in vitro* and *in vivo* approaches.