Characterization of partial-denitrification (PD) granular sludge producing nitrite: Effect of loading rates and...

The granule-based partial-denitrification (PD) reactor can achieve an efficient nitrite production for anammox process. In this study, the PD granules were successfully cultivated in a sequencing batch reactor (SBR), the physicochemical properties and microbial activities were characterized at increasing nitrate loading rate (NLR). Results indicated that high NLR benefited the PD for nitrite production, and a more dense and compact granule can be developed at the NLR of 0.24-0.48 Kg N/m³/d. Whereas the settling ability decreased when the NLR increased to 0.96 Kg N/m³/d, which was likely caused by the decrease of proteins (PN) in extracellular polymeric substances (EPS) content and the increase of loosely bound EPS (L-EPS) fraction. Besides, the characterization of size-fractionated PD granules revealed that a better settling ability was generally obtained with larger granules except for the size of d > 3.35 mm, which the settling velocity was reduced, likely attributed to the excess L-EPS fraction (56.3%) and presence of empty capsule inside granules. The smaller granules exhibited higher microbial activities due to the favorable mass transfer, the nitrate reduction rate was as high as 152.6 mg N/h/g VSS with granules of d < 1.0 mm. Results obtained in this study provided a better understanding of the properties of PD granules and would be helpful for the future development of granule-based PD reactor in achieving an efficient and stable nitrite production.