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Updated: Jan 27, 2026

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products
Published on: January 31, 2025
Method to Rapidly Enumerate Salmonella on Chicken Carcasses
S G Hawa1, G J Morrison1, G H Fleet1
1School of Food Technology, University of New South Wales, P.O. Box 1, Kensington, New South Wales, Australia 2033.
A new method rapidly enumerates Salmonella on chicken carcasses within 48 hours. This technique offers higher Salmonella counts and detection rates compared to traditional methods, improving food safety.
Area of Science:
- Food Microbiology
- Microbial Detection
- Food Safety
Background:
- Salmonella contamination on chicken carcasses poses a significant public health risk.
- Conventional methods for Salmonella detection are often time-consuming and may lack sensitivity.
Purpose of the Study:
- To develop and validate a rapid method for enumerating Salmonella on chicken carcasses.
- To improve the accuracy and speed of Salmonella detection in poultry products.
Main Methods:
- Carcass rinses were centrifuged to concentrate microbial cells.
- Selective isolation and differentiation using a novel dulcitol bile novobiocin agar.
- Biochemical confirmation with rapid lysine decarboxylase and ONPG tests.
Main Results:
- The developed method achieved confirmed Salmonella counts within 48 hours.
- Significantly higher Salmonella counts were obtained compared to conventional methods.
- Improved detection rates for Salmonella on chicken carcasses were observed.
Conclusions:
- The new method provides a rapid and more sensitive approach for Salmonella enumeration.
- This technique can enhance food safety protocols for poultry processing.
- Faster detection allows for quicker intervention and risk mitigation.
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