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Published on: August 23, 2019
Histamine Production by Psychrotrophic Pseudomonads Isolated from Tuna Fish
Elliot T Ryser1, Elmer H Marth1, Steve L Taylor1
1Department of Food Science and The Food Research Institute, University of Wisconsin-Madison, Madison, Wisconsin 53706.
Abstract:
Sixty isolates of psychrotrophic bacteria obtained from raw tuna fish were identified as Pseudomonas fluorescens , Pseudomonas putida and non-fluorescent Pseudomonas sp., and were tested for their ability to produce histamine. Following incubation in modified histidine decarboxylase broth, 28% of P. putida , 21% of P. fluorescens and 62% of the non-fluorescent Pseudomonas sp. isolates produced histamine. The maximum amount of histamine produced by a single isolate was 3.2 mg/100 ml, far below the minimum level of 50 mg/100 g believed necessary to induce symptoms of histamine toxicity.
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