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Related Experiment Videos

Pig skin as test substrate for evaluating topical antimicrobial activity.

L W Bush, L M Benson, J H White

    Journal of Clinical Microbiology
    |September 1, 1986
    PubMed
    Summary

    Pathogen-contaminated pig skin effectively models disinfectant efficacy for hand hygiene. Alcohol-based disinfectants reduce, but do not eliminate, pathogens, underscoring the need for frequent hand washing to control infections.

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    A CASE OF ALEUKAEMIC LYMPHATIC LEUKAEMIA.

    British medical journal·2010

    Area of Science:

    • Microbiology
    • Infection Control
    • Dermatology

    Background:

    • Evaluating skin disinfectants is crucial for infection control.
    • Existing models may not fully replicate clinical hand-washing scenarios.
    • Pathogen survival on skin necessitates effective decontamination strategies.

    Purpose of the Study:

    • To establish the feasibility of using pathogen-contaminated pig skin as a model for evaluating skin disinfectants.
    • To develop and validate a safe, convenient, and adaptable test methodology for hand hygiene.
    • To assess the efficacy of alcohol-based solutions against inoculated pathogens under controlled conditions.

    Main Methods:

    • Utilized pathogen-contaminated pig skin as a substrate for disinfectant testing.
    • Developed a methodology allowing control over contamination, treatment, and application variables.

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  • Employed contact imprint and stripping methods to quantify microbial transfer and reduction.
  • Validated the model using alcohol solutions of varying concentrations and types.

    • Main Results:

    • The pig skin model demonstrated feasibility, with microbial transfer ranging from 10-60% depending on contamination conditions.
    • Alcohol efficacy increased with concentration and followed the order: ethanol < isopropanol < n-propanol.
    • Disinfectant activity decreased under more severe test conditions (higher inoculum, longer incubation).
    • Alcohol treatments significantly reduced, but did not eradicate, inoculated pathogens, highlighting pathogen persistence.

    Conclusions:

    • Pathogen-contaminated pig skin serves as a viable model for assessing skin disinfectant performance.
    • The developed methodology is adaptable and allows for controlled simulation of clinical hand-washing.
    • Frequent hand washing is essential for infection control due to the potential for prolonged pathogen survival on skin.