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Counterstaining, Mounting, and Photographing Stained Cells.
Cold Spring Harbor Protocols
|April 3, 2019
Summary
For immunohistology, select counterstains and mounting media compatible with your detection method. Cell-staining results can be preserved using traditional film or modern digital storage techniques.
Area of Science:
- Histology
- Cell Biology
- Immunohistochemistry
Background:
- Immunohistochemistry (IHC) is a vital technique for visualizing cellular components.
- Proper selection of ancillary reagents is crucial for successful IHC staining and interpretation.
- Preservation of IHC results is necessary for documentation and further analysis.
Purpose of the Study:
- To highlight the importance of reagent compatibility in immunohistochemistry.
- To discuss methods for storing immunohistochemistry experimental outcomes.
Main Methods:
- Review of immunohistochemical staining protocols.
- Evaluation of reagent interactions in cell-staining experiments.
- Assessment of data storage options for microscopy results.
Main Results:
- Counterstains and mounting media must align with the specific detection system employed in immunohistology.
- Incompatibility can lead to artifacts, reduced signal, or loss of specific staining.
- Experimental outcomes from cell-staining can be recorded using photographic film or digital imaging.
Conclusions:
- Careful selection of counterstains and mounting media ensures reliable immunohistochemistry results.
- Both film and digital formats offer viable options for preserving cell-staining data.
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