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Updated: Jan 27, 2026

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin
Published on: January 8, 2016
An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging
Younghee Oh1,2,3,4, Yunhee Park1,2,3, Julia H Cho1,2,3
1Department of Neurobiology, Stanford University, Stanford, CA, USA.
Abstract:
Fluorescent indicators are used widely to visualize calcium dynamics downstream of membrane depolarization or G-protein-coupled receptor activation, but are poorly suited for non-invasive imaging in mammals. Here, we report a bright calcium-modulated bioluminescent indicator named Orange CaMBI (Orange Calcium-modulated Bioluminescent Indicator). Orange CaMBI reports calcium dynamics in single cells and, in the context of a transgenic mouse, reveals calcium oscillations in whole organs in an entirely non-invasive manner.
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