Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Computer analysis of radioligand data: advantages, problems, and pitfalls.

D Rodbard, R A Lutz, R A Cruciani

    NIDA Research Monograph
    |January 1, 1986
    PubMed
    Summary

    Mathematical modeling and nonlinear least-squares curve fitting offer objective ligand-binding data analysis. Careful interpretation, considering data quality and experimental design, ensures reliable results for biochemical and biophysical studies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Carbamazepine-loaded solid lipid nanoparticles and nanostructured lipid carriers: Physicochemical characterization and in vitro/in vivo evaluation.

    Colloids and surfaces. B, Biointerfaces·2018
    Same author

    Gastropod Egg Capsules and Their Contents From Deep-Sea Hydrothermal Vent Environments.

    The Biological bulletin·2018
    Same author

    RNA-sequencing of the brain transcriptome implicates dysregulation of neuroplasticity, circadian rhythms and GTPase binding in bipolar disorder.

    Molecular psychiatry·2014
    Same author

    Upregulation of α-synuclein during localized radiation therapy signals the association of cancer-related fatigue with the activation of inflammatory and neuroprotective pathways.

    Brain, behavior, and immunity·2012
    Same author

    Cooperative and redundant signaling of leukotriene B4 and leukotriene D4 in human monocytes.

    Allergy·2011
    Same author

    Glycaemic variability is associated with coronary artery calcium in men with Type 1 diabetes: the Coronary Artery Calcification in Type 1 Diabetes study.

    Diabetic medicine : a journal of the British Diabetic Association·2010

    Area of Science:

    • Biochemistry
    • Biophysics
    • Computational Biology

    Background:

    • Ligand-binding assays are crucial for understanding molecular interactions.
    • Traditional analysis methods can be subjective and lack reproducibility.
    • Mathematical modeling offers a systematic approach to data interpretation.

    Purpose of the Study:

    • To highlight the utility of mathematical modeling and nonlinear least-squares curve fitting for ligand-binding data analysis.
    • To outline potential pitfalls and limitations associated with these computational methods.
    • To emphasize the importance of rigorous data quality and experimental design.

    Main Methods:

    • Application of mathematical modeling techniques.
    • Utilizing nonlinear least-squares curve fitting for parameter estimation.

    Related Experiment Videos

  • Systematic formulation and testing of alternative hypotheses (models).

    • Main Results:

    • Modeling provides objective, reproducible, and consistent interpretation of ligand-binding data.
    • Estimates of goodness-of-fit and parameter uncertainty are generated.
    • Identified potential issues include data quality, experimental design, and statistical independence.

    Conclusions:

    • Mathematical modeling enhances the interpretation of ligand-binding data.
    • Awareness of limitations and potential biases is critical for accurate analysis.
    • Corroboration with independent evidence is essential for validating modeling results.