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Updated: Jan 26, 2026

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
Published on: October 25, 2018
Enzyme Immunoassay - Membrane Filter Method for Detection of Salmonellae in Foods
Jeffrey M Farber1, Pearl I Peterkin1, Anthony N Sharpe1
1Bureau of Microbial Hazards, Food Directorate, Health Protection Branch, Health and Welfare Canada, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2.
Abstract:
An enzyme-linked immunosorbent assay (ELISA) technique using a horseradish peroxidase-protein A-Spicer Edwards antiserum complex was developed for the detection of Salmonella colonies on membrane filters. In pure culture, 64 Salmonella species tested gave a positive reaction (purple stain). Of 22 naturally contaminated food samples, there was an exact correlation between the AOAC hydrophobic grid-membrane filter procedure and the ELISA technique (40.9% positives). This technique is simple, requires little equipment and can be completed in less than 2.5 h, thus allowing the detection of Salmonella spp. in foods within 48 h from initiation of sampling.
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