Distinct RNA recognition mechanisms in closely related LINEs from zebrafish

Long interspersed nuclear element (LINE) is known to be transposed by the reverse transcription using its RNA transcript. Recognition of the 3' stem-loop of LINE RNA by its reverse transcriptase (RT) is an important step of the retrotransposition. We focused on the RNA recognition by RT from two related LINEs, ZfL2-1 and ZfL2-2, from zebrafish. Previous study showed that RT from ZfL2-2 recognizes a single residue in the specific position of the RNA loop. In the present study, it was found that RT from ZfL2-1 recognizes the inserted stem-loop of ZfL2-1 RNA. Thus, these related RTs recognize the same region of LINE RNAs but discriminate them by different mechanism.