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Updated: Jan 26, 2026
09:05
A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
Published on: August 7, 2017
7.3K
Stromal prorenin receptor is critical for normal kidney development
Ihor V Yosypiv1, Maria Luisa S Sequeira-Lopez2, Renfang Song1
1Department of Pediatrics, Tulane University School of Medicine , New Orleans, Louisiana.
Summary
The prorenin receptor (PRR) in kidney stroma is vital for developing renal arteries and regulating nephron growth. Its absence causes neonatal death and impaired kidney development in mice.
Area of Science:
- Developmental biology
- Renal physiology
- Molecular genetics
Background:
- Kidney formation depends on stromal, ureteric bud, and cap mesenchyme interactions.
- Foxd1 marks renal stromal cells and is crucial for kidney development.
- The prorenin receptor (PRR) is essential for embryonic development, as global loss is lethal.
Purpose of the Study:
- Investigate the role of the prorenin receptor (PRR) in Foxd1-expressing renal stromal progenitors.
- Determine the impact of PRR deletion on kidney development and nephrogenesis.
Main Methods:
- Conditional deletion of the PRR in Foxd1+ stromal progenitors in mice (cKO).
- Analysis of kidney morphology, cell marker expression (Foxd1, Meis1, Six2), and vascular development.
- Assessment of renin expression in renal arterioles.
Main Results:
- Conditional PRR deletion in stromal progenitors caused neonatal mortality.
- Surviving cKO mice exhibited reduced stromal markers, impaired arterial development, fewer glomeruli, and delayed nephron differentiation.
- Reduced renin expression and thinner intrarenal arteries were observed in cKO mice.
Conclusions:
- Stromal PRR is critical for renal arterial tree development.
- PRR signaling in stroma influences renin-expressing cells and restricts nephron progenitor expansion.
- Stromal PRR is essential for coordinated nephrovascular morphogenesis in the mammalian kidney.
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