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[Various ways of standardizing and unifying biological methods of determining the activity of antibiotics]
Antibiotiki I Meditsinskaia Biotekhnologiia = Antibiotics and Medical Biotechnology
|July 1, 1986
Abstract:
It was shown possible to use nutrient media containing only salt components and agar-agar for determination of biological activity of tetracyclines (tetracycline, morphocycline, oxytetracycline and chlortetracycline), erythromycin and oleandomycin. A uniform nutrient medium containing ammonium chloride, trisubstituted sodium citrate, disubstituted sodium phosphate and agar-agar was developed. Glucose is added to this medium simultaneously with the test microbe, B. subtilis ATCC 6633. The medium of the above composition provided readily reproducible results.