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Updated: Jan 26, 2026

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
Published on: August 5, 2020
Identification of Novel lincRNA and Co-Expression Network Analysis Using RNA-Sequencing Data in Plants
Song Qi1,2, Shamima Akter2, Song Li3
1Ph.D. Program in Genetics, Bioinformatics and Computational Biology, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
Abstract:
Long intergenic noncoding RNA (lincRNA) plays important biological functions in plants. Identification and annotation of lincRNA in plants largely rely on RNA sequencing followed by computational analysis. In this protocol, we describe a multistep computational pipeline for lincRNA identification using RNA-sequencing data. This pipeline can also construct co-expression network that is made of both lincRNA and mRNA genes. The co-expression network generated by this pipeline can be used to provide putative annotation of lincRNAs that have no known biological functions.
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