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Development of a robotics platform for automated multi-ionization mass spectrometry
Santosh Karki1,2, Anil K Meher1,2, Ellen D Inutan1,2
1Department of Chemistry, Wayne State University, Detroit, MI, USA.
Rapid Communications in Mass Spectrometry : RCM
|April 6, 2019
Summary
A new automated platform integrates multiple ionization methods for mass spectrometry, improving compound detection in complex samples. This versatile system enhances analysis of peptides, drugs, and biological extracts.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
Background:
- Automated platforms enhance mass spectrometry by enabling diverse ionization techniques.
- Coupling with commercial mass spectrometers improves compound coverage in complex mixtures.
Purpose of the Study:
- To demonstrate the versatility of a multi-ionization automated platform.
- To evaluate its performance with different ionization methods (ESI, SAI, MAI) and mass spectrometers.
Main Methods:
- An automated platform samples from well plates and surfaces.
- It interfaces with Thermo Fisher and Waters mass spectrometers.
- Utilizes electrospray ionization (ESI), solvent-assisted ionization (SAI), and matrix-assisted ionization (MAI).
Main Results:
- Significant differences in ionization efficiency observed across methods for various compound classes.
- Rapid analysis (1 min/sample) of antibiotic mixtures achieved using MAI and ESI.
- Successful analysis of bacterial extracts and surface samples (TLC, tissue).
Conclusions:
- The automated platform supports solid and solution matrix introduction for diverse ionization methods.
- Combining ionization techniques broadens compound applicability, especially for complex biological samples.
- The platform is adaptable for most commercial mass spectrometers.
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