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Updated: Jan 26, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Novel detection platform for insulin based on dual-cycle signal amplification by Exonuclease III
Chen Liu1, Jialun Han1, Jingjing Zhang1
1State Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials and Chemical Engineering, Hainan University, Haikou 570228, PR China.
Abstract:
A novel detection platform based on Exonuclease III dual-cycle signal amplification with graphene oxide as quenching agent and DNA labelling as recognition element has been developed for the fluorometric determination of insulin. Specific DNA sequences were cut by Exo III to control the fluorescence recovery of the system. Fluorescence intensity analysis was carried out by fluorescence spectrophotometer to obtain the fluorescence intensity corresponding to different concentrations of insulin. When insulin is added to the system, insulin binds to the adaptor and the fluorescence is quenched, whereas in the absence of insulin the fluorescence is restored. The sensor platform achieved multiple signal amplification affording rapid and sensitive detection of insulin with a detection range of 0.048-2.15 U/ml. The methodology is rapid, sensitive, of low cost and convenient to perform and has potential for routine screening of insulin in blood, and also has prospects for the detection of other proteins and biomolecules.
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