Related Experiment Video
Updated: Jan 26, 2026
11:34
Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
Published on: December 5, 2017
13.1K
Concise approach for screening long non-coding RNAs functionally linked to human breast cancer associated genes
Tae-Jin Shin1, Kang-Hoon Lee1, Hyun-Min Cho1
1Department of Biochemistry, BK21 Plus and Research Institute for Veterinary Science, School of Veterinary Medicine, Seoul National University, Seoul, South Korea.
Experimental and Molecular Pathology
|April 7, 2019
Summary
This study identifies novel long non-coding RNAs (lncRNAs) associated with breast cancer by analyzing their expression patterns alongside breast cancer-associated genes (BCAGs). One lncRNA, LOC105371849, was found to regulate HEATR6 expression, suggesting potential diagnostic and therapeutic roles.
Area of Science:
- Oncology
- Genomics
- Molecular Biology
Background:
- Aberrant gene expression is linked to various cancers, with long non-coding RNAs (lncRNAs) emerging as key regulators.
- lncRNAs exhibit tissue-specific expression and play roles in cell cancerization, including regulating tumor suppressors.
Purpose of the Study:
- To develop an efficient method for identifying lncRNAs associated with breast cancer.
- To analyze the expression patterns of lncRNAs adjacent to breast cancer-associated genes (BCAGs) in human breast cancer cell lines.
Main Methods:
- Literature review to identify 337 BCAGs.
- Bioinformatic analysis to locate 121 lncRNAs near BCAGs.
- Expression profiling of lncRNAs in human breast cancer cell lines.
Main Results:
- Twenty lncRNAs showed detectable expression in breast cancer cell lines.
- Three lncRNAs (two up-regulated, one down-regulated) correlated with adjacent BCAGs.
- Knockdown of lncRNA LOC105371849 decreased HEATR6 mRNA expression in MCF7 cells.
Conclusions:
- This study enhances understanding of lncRNA biological roles in breast cancer.
- Identified lncRNAs may serve as potential diagnostic and therapeutic targets for breast cancer.
- The association between LOC105371849 and HEATR6 provides a new avenue for breast cancer research.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
9.8K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.8K
02:39lncRNA - Long Non-coding RNAs
3.6K
3.6K
02:30siRNA - Small Interfering RNAs
18.5K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.5K
02:57piRNA - Piwi-interacting RNAs
7.6K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.6K
01:42What is Gene Expression?
195.3K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
195.3K
02:04Covalently Linked Protein Regulators
8.9K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
8.9K