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Delta-like Protein 3 Prevalence in Small Cell Lung Cancer and DLL3 (SP347) Assay Characteristics
Richard S P Huang1, Burton F Holmes1, Courtney Powell1
1From the Department of Companion Diagnostics, Ventana Medical Systems, Tucson, Arizona.
Archives of Pathology & Laboratory Medicine
|April 9, 2019
Summary
Delta-like protein 3 (DLL3) is expressed in 76.4% of small cell lung cancer cases. The VENTANA DLL3 (SP347) Assay effectively characterizes DLL3 expression, aiding in diagnostic assessments.
Area of Science:
- Oncology
- Immunohistochemistry
- Molecular Pathology
Background:
- Delta-like protein 3 (DLL3) is a key protein in the Notch pathway.
- DLL3 expression is relevant in small cell lung cancer (SCLC).
Purpose of the Study:
- To determine DLL3 prevalence in SCLC using the VENTANA DLL3 (SP347) Assay.
- To characterize DLL3 staining patterns in SCLC.
- To evaluate the assay's immunoreactivity in various tissues.
Main Methods:
- Procurement of formalin-fixed, paraffin-embedded SCLC specimens and tissue microarrays.
- Staining of specimens using the VENTANA DLL3 (SP347) assay.
- Microscopic examination for prevalence, staining characteristics, and immunoreactivity.
Main Results:
- DLL3 expression was observed in 76.4% (1040/1362) of SCLC specimens.
- Staining patterns included cytoplasmic and/or membranous, with homogenous/heterogeneous and partial/circumferential granular staining of varied intensities.
- Immunoreactivity was also detected in other neoplastic and nonneoplastic tissues.
Conclusions:
- The study provides a detailed profile of DLL3 staining characteristics in SCLC.
- The VENTANA DLL3 (SP347) Assay is effective for assessing DLL3 expression levels in SCLC.
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