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Updated: Jan 26, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
Published on: November 8, 2016
ICMSF Methods Studies. XVI. Comparison of Salt Polymyxin Broth with Glucose Salt Teepol Broth for Enumerating Vibrio
R Sakazaki1, H Pivnick1, G Jarvis1
1National Institute of Health, 10-35 Kamiosaki 2-chome, Shinagawa-ku, Tokyo, Japan; Department of National Health and Welfare, Tunney's Pasture, Ottawa, Canada, K1A OL2; Shizuoka Public Health Laboratory, 3-6-2 Takajo-Cho, Shizuoka 420, Japan; PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, SP4 OJG, England; University of Georgia College of Agriculture, Experiment, Georgia 30212; University of Maryland, College Park, Maryland 20742; University of Delaware, Newark, Delaware 19711; Public Health Research Institute of Kobe, 6, 4 chome, Minatojima-Nakamachi, Chuo-ku, Kobe 650, Japan; Tokyo Metropolitan Institute of Public Health, 3-24-1 Hyakunin-Cho, Shinjuku-ku, Tokyo 160, Japan; Public Health Laboratory of Kanagawa Prefecture, 52-2 Nakao-Cho, Asahi-ku, Yokohama 241, Japan; Food and Drug Administration, Cincinnati, Ohio 45226; and Texas A&M University, College Station, Texas 77843.
Abstract:
Two media, glucose salt teepol broth (GSTB) and salt polymyxin broth (SPB), were compared for their efficacy in enumerating Vibrio parahaemolyticus in naturally contaminated samples using the most probable number (MPN) procedure. Eleven laboratories in four countries participated, six of them using two analysts. One hundred ninety-six of 335 samples were found to contain V. parahaemolyticus . Neither enrichment medium was superior, and both media appeared necessary to prevent false-negative results. There was a high (52.5%) percentage of MPN patterns that were improbable with respect to Poisson distribution in those MPN tubes that yielded V. parahaemolyticus . Suspect colonies that developed on thiosulfate citrate bile salts sucrose (TCBS) plates streaked from GSTB were confirmed (1,438/1,929 = 75%) about as frequently as when streaked from SPB (2,155/3,038 = 71%).
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