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Published on: May 11, 2019
Evaluation of Methods for Extraction of Enteric Virus from Louisiana Oysters
Mary Townsend Cole1, Marilyn B Kilgen1, Cameron R Hackney1
1Department of Food Science, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803 and Department of Biology, Nicholls State University, Thibodaux, Louisiana 70803.
Abstract:
Six techniques were evaluated for recovery of poliovirus from Louisiana oysters. The methods were compared for percent recovery rates, toxicity, ease of extraction, bacterial contamination, and final volume of oyster concentrate. Oyster samples were contaminated with 30-40 plaque forming units of Poliovirus type 1 and processed by six variations of adsorption-elution-precipitation and elution-precipitation methods. The method developed by Ellender et al. (Natural enterovirus and fecal coliform contamination of gulf coast oysters. J. Food Prot. 43:105-110) was judged to be the preferred method for gulf coast oysters.
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