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Published on: March 7, 2018
Drying Enhances Signal Intensities for Global GC⁻MS Metabolomics
1Max-Planck Institute for Marine Microbiology, Celsiusstr. 1, 28359 Bremen, Germany. mliebeke@mpi-bremen.de.
Abstract:
We report here that a straightforward change of the standard derivatization procedure for GC⁻MS metabolomics is leading to a strong increase in metabolite signal intensity. Drying samples between methoxymation and trimethylsilylation significantly increased signals by two- to tenfold in extracts of yeast cells, plant and animal tissue, and human urine. This easy step reduces the cost of sample material and the need for expensive new hardware.
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